Tuesday, April 26, 2011

EXPERIMENT 2:- Biopesticides Comparative Analysis of Extracts Formulation Jeremin Oil Pelargonium radula as Insect Repellent

Title
The effectiveness of  different types of   biopesticide products on the  repellent activity against flies.

Aim
To determine the effectiveness of different types of biopesticide products on the repellent activity against flies

Hypothesis
The jeremin oil has the mostrepellent activity than the other different types of biopesticides

Variables
Manipulated
Types ofbiopesticide products [jeremin oil, Serai Wangi Haniff, and pine oil]

Responding
Repellent activity against flies

Fixed
Size of aquarium

Apparatus
Aquarium, Petri dish

Materials
10ml of 3% Jeremin oil, 10ml of distilled water, 10ml of Serai Wangi Haniff products,
10ml of pine oil, glucose solution, household flies

Diagram 5.0: Aquariums which contain flies

Procedure
1.      Theaquarium was divided into three zones which was 13 cm each zone.
2.      The zones of aquarium were labelled as A,B and C.
3.      The aquarium was placed in normal position in a well-ventilated room.
4.      The glucose solution was placed in the middle of aquarium as the food to feed the flies.
5.      15 flies were placed into the aquarium.
6.      10ml of jeremin oil was placed in the petri dish and it was placed at the end of zone A.

Diagram 6.0 Experimental set up of the experiment

7.      The flies were observed after 10th minutes, 30th minutes and 50th minutes.
8.      The experiment was repeated three times to get average number of repelled flies.
9.      Step 1-7 was repeated by using 10ml of distilled water, 10ml of Serai Wangi Haniff and 10ml of pine oil.
10.  The data obtained was tabulated


5.0 RESULTS

Table 1.0: The effect of jeremin oil on the repellent activity against flies

Time
(min)


Reading
10
30
50
A
B
C
DIED
A
B
C
DIED
A
B
C
DIED
1st
3
4
6
2
1
4
8
2
1
2
9
3
2nd
2
3
8
2
1
2
8
3
1
1
11
2
3rd
3
3
8
1
2
2
9
2
2
1
10
2
Average
3
3
7
2
1
3
8
2
1
1
10
2


Table 2.0: The effect of pine oilon the repellent activity against flies

Time
(min)


Reading
10
30
50
A
B
C
DIED
A
B
C
DIED
A
B
C
DIED
1st
7
3
3
2
3
4
2
6
4
2
2
7
2nd
8
2
1
4
5
5
0
5
4
3
1
8
3rd
7
2
2
3
5
4
1
5
5
3
1
6
Average
7
2
2
3
4
4
1
5
4
3
2
7


Table 3.0: The effect of Serai Wangi Haniff the repellent activity against flies

Time
(min)


Reading
10
30
50
A
B
C
DIED
A
B
C
DIED
A
B
C
DIED
1st
1
1
11
2
2
1
11
3
3
2
7
3
2nd
2
2
10
1
1
3
9
3
4
1
6
4
3rd
2
1
10
2
2
1
8
4
3
1
7
4
Average
2
1
10
2
2
2
9
3
3
1
7
4


Table 4.0: Controlled experiment using distilled water

Time
(min)


Reading
10
30
50
A
B
C
DIED
A
B
C
DIED
A
B
C
DIED
1st
5
7
3
0
6
5
4
0
5
4
6
0
2nd
6
8
1
0
4
8
2
1
6
6
2
1
3rd
7
6
2
0
5
9
1
0
5
7
1
2
Average
6
7
2
0
5
7
2
0
5
6
3
1






DATA ANALYSIS
                Based on the experiment above, pine oil seems not effective to drive away flies as it can only repel the average of 1 housefly. Serai Wangi Haniff repelled many flies at the early experiment which was in the first 10 minutes as it was able to repel the average of 10 flies. However, the number of flies that was repelled by this biopesticide decreases with the increases of time taken. After the 50th minutes, the average number of flies that was repelled by Serai Wangi Haniff decreases gradually to 7 flies. For jeremin oil’s results, the effectiveness of this biopesticide increased as time taken. In the first 10 minutes, it repelled the average of 7 flies but after 50 minutes, the average number of flies which was repelled by Jeremin oil increased to 10.0. My hypothesis is jeremin oil is the best biopesticide products as it can repel the flies in the long period.






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